NDLI: Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.

Content Provider	Springer Nature Link
Author	Regalado, J. J. Carmona Martín, E. Castro, P. More, R. Gil, J. Encina, C. L.
Copyright Year	2015
Abstract	Polyploid plants have been induced in different Asparagus officinalis L. breeding programs in order to obtain plants with improved agronomical traits, such as large spear diameter or segregation ratios with a higher number of males. The polyploidization methods can produce somaclonal variation in the polyploid plants obtained and, therefore, unwanted changes in the agronomical traits of the initial elite plants. We used two different polyploidization methods to induce polyploid plants from diploid genotypes of commercial varieties and tetraploid genotypes of the Spanish landrace “Morado de Huétor”. The first method was the culture of rhizome buds in the medium ARBM-3 (Asparagus Rhizome Bud Medium), supplemented with different concentrations of colchicine (0.1–0.75 g l$^{−1}$) for 10 and 20 days. The best polyploidization rate obtained was 25 % (0.5 g l$^{−1}$ colchicine for 10 days). The second method was the regeneration of polyploid plants from callus culture, resulting in a polyploidization rate of 40 and 12.5 % for the diploid genotype CM077 and the tetraploid genotype HT156, respectively. Additionally, we have developed a new protocol to separate the mixoploids generated into their different genetic components, obtaining plants with a unique ploidy level. EST-SSRs markers were employed to analyze the genetic stability of polyploidy plants. Somaclonal variation was not detected for polyploidy plants obtained through the culture of rhizome bud explants. Therefore, these polyploid plants should maintain the agronomical traits of the initial elite plants. However, somaclonal variation was detected in the polyploid plants regenerated from callus culture.
Starting Page	31
Ending Page	44
Page Count	14
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	122
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-02-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colchicine Rhizome buds Endoreduplication EST-SSRs Organogenesis Separation of mixoploids Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Ploidy distribution in the explant tissue and the calluses induced during the initial stage of internode segment culture of Asparagus officinalis L.

In vitro induction of autooctoploid asparagus genotypes

Production of compact petunias through polyploidization

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

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A new and efficient micropropagation method and its breeding applications in Asparagus genera

In vitro induction of tetraploids in Arachis paraguariensis

Somaclonal and chromosomal effects of genotype, ploidy and culture duration in Asparagus officinalis L.

Study of the somaclonal variation produced by different methods of polyploidization in Asparagus officinalis L.

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Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

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