NDLI: Genetic homogeneity of guava plants derived from somatic embryogenesis using SSR and ISSR markers

Content Provider	Springer Nature Link
Author	Rai, Maj K. Phulwaria, Mahendra Gupta, Amit K. Shekhawat, N. S. Jaiswal, U.
Copyright Year	2012
Abstract	To evaluate genetic homogeneity of 1-year-old guava (Psidium guajava L.) plants developed from in vitro somatic embryogenesis, DNA from leaf tissues of seven randomly selected plants along with the mother plant, was isolated and subjected to molecular analysis. A total of six Simple Sequence Repeat (SSR) primer pairs, producing reproducible and clear bands ranging from 100 to 300 bp in size, resulted in amplification of single band (allele), corresponding homozygous individuals. Moreover, of 10 different inter-simple sequence repeat (ISSR) primers screened, six produced resolvable, reproducible and scorable bands. All these ISSRs produced a total of 25 bands, ranging between 300 and 1,200 bp length, and the number of scorable bands, for each primer varied from three to six with an average of 4.1 bands per primer. The amplification products were monomorphic across all the micropropagated plants produced by all SSR and ISSR primers applied. The monomorphic banding pattern in micropropagated plants and the mother plant confirms the genetic homogeneity of the in vitro raised plants and demonstrates the reliability of our in vitro propagation system for guava.
Starting Page	259
Ending Page	264
Page Count	6
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	111
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-06-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genetic fidelity Micropropagation Molecular markers Somaclonal variation Somatic embryogenesis Plant Sciences Plant Genetics & Genomics Plant Physiology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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