NDLI: Enhanced vincamine production in selected tryptophan-overproducing shoots of Vinca minor

Content Provider	Springer Nature Link
Author	Verma, Priyanka Mathur, Ajay K. Shanker, Karuna
Copyright Year	2012
Abstract	Vinca minor is the sole source of vincamine, an alkaloid known to be used in a variety of cerebral disorders. Three stable variant shoot lines (V10, V20 and V30) with tolerance thresholds of 10, 20 and 30 mg/l 5-methyltryptophan (5-MT; analogue of tryptophan), respectively, were selected. These lines showed twofold to threefold increase in tryptophan content and 1.5- to 2-fold increment in the total alkaloids in comparison to the wild line shoots. A maximum of 16-fold enhancement in vincamine production was recorded in V30 line followed by eightfold in V20 line. Inter simple sequence repeat (ISSR)-PCR amplification of all the three lines showed total of 65 bands; out of which 60 were monomorphic (92.3 %) and 5 were polymorphic (7.7 %). Tryptophan being a limiting factor in the indole alkaloid pathway plays a crucial role in modulating the flux towards vincamine production and its over-production positively resulted into enhanced vincamine production.
Starting Page	239
Ending Page	245
Page Count	7
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	111
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-06-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vinca minor Tryptophan Vincamine 5-methyl tryptophan ISSR Plant Sciences Plant Genetics & Genomics Plant Physiology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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