NDLI: Detection of DNA changes in somaclonal mutants of rice using SSR markers and transposon display

Content Provider	Springer Nature Link
Author	Gao, Dong Ying Vallejo, Veronica A. He, Bing Gai, Yun Chao Sun, Li Hua
Copyright Year	2009
Abstract	Somaclonal variation detected in plant tissues grown in vitro can be heritable, and thus provides an opportunity for plant breeders and geneticists to generate novel variants. However, incidence of somaclonal variation is problematic for plant transformation efforts and for micropropagation of true-to-type clones. The origin of somaclonal variation is still not well understood. In this study, 120 SSR markers, distributed across all 12 chromosomes of rice, have been used to analyze eight somaclonal mutants derived from seven different cultivars. Of these, 13 SSRs have detected polymorphisms between the bacterial blight resistant mutant HX-3 and its wild-type Minghui 63. While, ten SSRs have revealed differences between a purple sheath mutant, Z418, and the wild-type C418. None of the SSRs have been able to distinguish between tall and dwarf mutants, 02428h and A418, and their wild-type counterparts, respectively. Interestingly, six SSRs have identified differences in at least three mutant lines and their corresponding wild-type genotypes. These results have suggested that some SSR markers in the rice genome may detect higher numbers of polymorphisms than others. In addition, a transposon display (TD) of five active rice transposons, Tos17, Karma, mPing, nDart and dTok, has been conducted to evaluate DNA changes of eight mutants. Some mutant lines, such as HX-3 and Z418, have exhibited differences from their corresponding wild-type genotypes in TDs with two transposons. This has indicated that new insertions of transposons are involved in somaclonal variation derived from tissue culture. Taken together, these results suggest that multiple molecular mechanisms are responsible for somaclonal variation detected in tissue culture of rice.
Starting Page	187
Ending Page	196
Page Count	10
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	98
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-06-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Somaclonal variation SSR Transposon display Rice Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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