NDLI: Cyclic secondary somatic embryogenesis and efficient plant regeneration in mountain ash (Sorbus pohuashanensis)

Content Provider	Springer Nature Link
Author	Yang, Ling Wang, Jianan Bian, Lei Li, Yuhua Shen, Hailong
Copyright Year	2012
Abstract	An efficient protocol for secondary somatic embryogenesis in mountain ash is reported. Secondary somatic embryos (SSEs), initially obtained from primary embryos, were proliferated and maintained for more than 2 years via cyclic secondary somatic embryogenesis. SSEs were produced on the surfaces of cotyledons and radicles of maternal somatic embryos. Histological observations of the various stages of SSE development revealed four typical stages: globular, heart-shaped, torpedo, and cotyledon. Addition of a low concentration of naphthaleneacetic acid (NAA) to Murashige and Skoog (MS) medium resulted in the induction of SSEs, but addition of 2,4-dichlorophenoxyacetic acid (2,4-d) to MS medium decreased SSE formation. Addition of casein hydrolysate (CH) to MS medium promoted induction of SSEs. Cotyledonary SSEs were cultured on MS medium with 20–60 g L$^{−1}$ sucrose under light for 1 month until maturation. After transferred to MS medium containing either 0.06 μM NAA or 0.15 μM indole-3-butyric acid in the light, over 50 % of the mature SSEs developed into plantlets. Addition of 1.0 g L$^{−1}$ activated charcoal was beneficial for SSE germination (over 60 %). At 18 °C, over 90 % of the germinated SSEs converted to plantlets on ½ MS (half-strength of MS macroelements) with 1.8 μM NAA under light. Plantlets acclimatized successfully to ex vitro conditions and field plants developed with normal phenotypes.
Starting Page	173
Ending Page	182
Page Count	10
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	111
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-06-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mountain ash Repetitive embryogenesis Somatic plants In vitro conservation Plant Sciences Plant Genetics & Genomics Plant Physiology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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