NDLI: Identification of resistance-associated proteins in closely-related maize lines varying in aflatoxin accumulation

Content Provider	Springer Nature Link
Author	Chen, Zhi Yuan Brown, Robert L. Menkir, Abebe Cleveland, Thomas E.
Copyright Year	2011
Abstract	Aspergillus flavus infection of maize and subsequent contamination with carcinogenic aflatoxins poses serious health concerns, especially in developing countries. Maize lines resistant to A. flavus infection have been identified; however, the development of commercially-useful aflatoxin-resistant maize lines has been hindered due to a lack of breeding markers. To identify maize resistance-associated proteins (RAPs) as potential markers for breeding, 52 BC1S4 lines developed from crosses between five African maize inbreds and five temperate aflatoxin-resistant lines were screened using the kernel screening assay. Five pairs of closely-related lines that had 75–94% genetic similarity within each pair and which varied within each pair in aflatoxin accumulation were selected for proteomic investigation. Kernel embryo and endosperm protein profile differences within the pair and across pairs were compared using two-dimensional polyacrylamide gel electrophoresis. Differentially expressed (≥1.5-fold) RAPs were sequenced through tandem mass spectrometry and were identified as antifungal, stress-related, storage or regulatory proteins. Sequence homology analysis highlighted several proteins in maize that confer resistance to A. flavus infection and/or aflatoxin production.
Starting Page	53
Ending Page	68
Page Count	16
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	30
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Two-dimensional gel electrophoresis Corn Protein profile comparison Aflatoxin resistance Antifungal protein Stress-related protein Plant Pathology Plant Sciences Biotechnology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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