NDLI: Molecular characterization and diversity of the Pina and Pinb genes in cultivated and wild diploid wheat

Content Provider	Springer Nature Link
Author	Guzmán, C. Caballero, L. Martín, M. A. Alvarez, J. B.
Copyright Year	2011
Abstract	Grain hardness is one of the most important characteristics of wheat quality. Soft endosperm is associated with the presence of two proteins in the wild form, puroindoline a and b. The puroindoline genes and their derived proteins are present in the putative wheat diploid ancestors which are thought to be the donors of the A, B and D genomes in common and durum wheat. In this study, we investigated the variability of grain hardness in einkorn, along with the nucleotide diversity of Pina and Pinb genes in a collection of einkorn wheat and T. urartu, in addition to studying the neutrality and linkage disequilibrium of these genes. Various alleles were detected for Pina and Pinb genes including three novel alleles for the Pinb locus: Pinb-A $^{ m }$ 1i, Pinb-A $^{ m }$ 1j and Pinb-A $^{ m }$ 1k. Some differences were found in grain hardness between the different genotypes. The neutrality test showed a different pattern of variation between the two Pin genes. The genetic analysis of a diploid wheat collection has demonstrated that these species are a potential source of novel puroindoline variants. Our data suggest that, although further studies must be carried out, these variants could be used to expand the range of grain texture in durum and common wheat, which would permit the development of new materials adapted to novel uses in the baking and pasta industry.
Starting Page	69
Ending Page	78
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	30
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Einkorn wheat Grain hardness Kernel texture Puroindolines Plant Pathology Plant Sciences Biotechnology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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