NDLI: New PCR-based sequence-tagged site marker for bacterial blight resistance gene Xa38 of rice

Content Provider	Springer Nature Link
Author	Bhasin, Hemal Bhatia, Dharminder Raghuvanshi, Saurabh Lore, Jagjit S. Sahi, Gurpreet K. Kaur, Baljit Vikal, Yogesh Singh, Kuldeep
Copyright Year	2011
Abstract	Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae, is a major disease of rice managed largely through the deployment of resistance genes. Xa38, a BB resistance gene identified from Oryza nivara acc. IRGC 81825, was mapped on chromosome 4L in a 38.4-kb region. The closely linked markers for this gene, identified earlier, were simple sequence repeat marker RM17499 and sequence-tagged site markers developed from loci Os04g53060 and Os04g53120. Marker Os04g53060 is dominant while the other two markers show smaller size differences difficult to resolve accurately on agarose gel. Based on gene annotation, three nucleotide binding site–leucine-rich repeat genes present in the target region were cloned from O. nivara and sequenced. One of the loci, LOC_Os04g53050, had a 48-base-pair deletion in O. nivara acc. IRGC 81825 compared to the cultivated rice. Primers were designed around the deletion and the resulting marker is codominant and easy to score in agarose gel. The newly designed marker co-segregated with Xa38, amplifying products of 269 bp in O. nivara and 317 bp in cultivated rice. This marker could be more useful for marker-assisted selection than ones reported earlier.
Starting Page	607
Ending Page	611
Page Count	5
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	30
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-10-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oryza sativa Oryza nivara Bacterial blight Xa38 STS marker Plant Pathology Plant Sciences Biotechnology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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