NDLI: Large-scale development of expressed sequence tag-derived simple sequence repeat markers and diversity analysis in Arachis spp.

Content Provider	Springer Nature Link
Author	Koilkonda, Padmalatha Sato, Shusei Tabata, Satoshi Shirasawa, Kenta Hirakawa, Hideki Sakai, Hiroe Sasamoto, Shigemi Watanabe, Akiko Wada, Tsuyuko Kishida, Yoshie Tsuruoka, Hisa Fujishiro, Tsunakazu Yamada, Manabu Kohara, Mitsuyo Suzuki, Shigeru Hasegawa, Makoto Kiyoshima, Hiroyuki Isobe, Sachiko
Copyright Year	2011
Abstract	Large-scale development of expressed sequence tag simple sequence repeat (EST-SSR) markers was performed in peanut (Arachis hypogaea L.) to obtain more informative genetic markers. A total of 10,102 potential non-redundant EST sequences, including 3,445 contigs and 6,657 singletons, were generated from cDNA libraries of the gynophore, roots, leaves and seedlings. A total of 3,187 primer pairs were designed on flanking regions of SSRs, some of which allowed one and two base mismatches. Among the 3,187 markers generated, 2,540 (80%) were trinucleotide repeats, 302 (9%) were dinucleotide repeats, and 345 (11%) were tetranucleotide repeats. Pre-polymorphic analyses of 24 Arachis accessions were performed using 10% polyacrylamide gels. A total of 1,571 EST-SSR markers showing clear polymorphisms were selected for further polymorphic analysis with a Fluoro-fragment Analyzer. The 16 Arachis accessions examined included cultivated peanut varieties as well as diploid species with the A or B genome. Altogether 1,281 (81.5%) of the 1,571 markers were polymorphic among the 16 accessions, and 366 (23.3%) were polymorphic among the 12 cultivated varieties. Diversity analysis was performed and the genotypes of all 16 Arachis accessions showed similarity coefficients ranging from 0.37 to 0.97.
Starting Page	125
Ending Page	138
Page Count	14
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	30
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arachis spp. EST-SSR marker Polymorphic analysis Genetic diversity Plant Pathology Plant Sciences Biotechnology Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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