NDLI: Detachment strength of human osteoblasts cultured on hydroxyapatite with various surface roughness. Contribution of integrin subunits

Content Provider	Springer Nature Link
Author	Kokkis, Petros A. Koutsoukos, Petros G. Deligianni, Despina D.
Copyright Year	2012
Abstract	Hydroxyapatite (HA) has been widely used as a bone substitute in dental, maxillofacial and orthopaedic surgery and as osteoconductive bone substitute or precoating of pedicle screws and cages in spine surgery. The aim of the present study was to investigate the osteoblastic adhesion strength on HA substrata with different surface topography and biochemistry (pre-adsorption of fibronectin) after blocking of specific integrin subunits with monoclonal antibodies. Stoichiometric HA was prepared by precipitation followed by ageing and characterized by SEM, EDX, powder XRD, Raman spectroscopy, TGA, and specific surface area analysis. Human bone marrow derived osteoblasts were cultured on HA disc-shaped substrata which were sintered and polished resulting in two surface roughness grades. For attachment evaluation, cells were incubated with monoclonal antibodies and seeded for 2 h on the substrata. Cell detachment strength was determined using a rotating disc device. Cell detachment strength was surface roughness, fibronectin preadsorption and intergin subunit sensitive.
Starting Page	1489
Ending Page	1498
Page Count	10
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	23
Issue Number	6
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2012-04-08
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Metallic Materials Surfaces and Interfaces, Thin Films Polymer Sciences Biomaterials Characterization and Evaluation of Materials Ceramics, Glass, Composites, Natural Methods
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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