NDLI: Heterologous expression of a Volvox cell adhesion molecule causes flocculation in Chlamydomonas reinhardtii

Content Provider	Springer Nature Link
Author	Lowder, Levi G. Herbert, Stephen K.
Copyright Year	2014
Abstract	Harvesting microalgae at large scales is a significant barrier to economically feasible production of biofuels and other low-cost commodities from microalgal biomass. We have tested a strategy for reducing the costs of microalgal harvest by generating transgenic strains of Chlamydomonas reinhardtii that express a heterologous cellular adhesion molecule, Algal-CAM, from the multicellular green alga Volvox carteri. Constitutive expression of heterologous Algal-CAM causes Chlamydomonas unicells to adhere together and settle out of suspension much more rapidly than controls. Immunoblotting shows the heterologous Algal-CAM to be present in the extracellular matrix of Chlamydomonas transformants and possibly cross-linked with native glycoproteins there. We define this form of cell–cell adhesion as genetically engineered (GE) flocculation to distinguish it from other cell flocculation strategies. Future development of this trait can include making expression of Algal-CAM inducible for controlled timing of GE flocculation and exploring regulated expression of additional cell adhesion molecules from the many other multicellular relatives of Chlamydomonas. Advanced forms of this technology could lead to production of novel biomaterials from single-celled algae by controlled expression of diverse cell adhesion molecules with different cross-linking properties.
Starting Page	721
Ending Page	731
Page Count	11
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	27
Issue Number	2
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-09-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flocculation Cell adhesion molecule Extracellular matrix Biotechnology Chlamydomonas Volvox Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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