NDLI: Metabolic modeling of Chlamydomonas reinhardtii: energy requirements for photoautotrophic growth and maintenance

Content Provider	Springer Nature Link
Author	Kliphuis, Anna M. J. Klok, Anne J. Martens, Dirk E. Lamers, Packo P. Janssen, Marcel Wijffels, René H.
Copyright Year	2011
Abstract	In this study, a metabolic network describing the primary metabolism of Chlamydomonas reinhardtii was constructed. By performing chemostat experiments at different growth rates, energy parameters for maintenance and biomass formation were determined. The chemostats were run at low irradiances resulting in a high biomass yield on light of 1.25 g mol$^{−1}$. The ATP requirement for biomass formation from biopolymers (K $_{ x }$) was determined to be 109 mmol g$^{−1}$ (18.9 mol mol$^{−1}$) and the maintenance requirement (m $_{ATP}$) was determined to be 2.85 mmol g$^{−1}$ h$^{−1}$. With these energy requirements included in the metabolic network, the network accurately describes the primary metabolism of C. reinhardtii and can be used for modeling of C. reinhardtii growth and metabolism. Simulations confirmed that cultivating microalgae at low growth rates is unfavorable because of the high maintenance requirements which result in low biomass yields. At high light supply rates, biomass yields will decrease due to light saturation effects. Thus, to optimize biomass yield on light energy in photobioreactors, an optimum between low and high light supply rates should be found. These simulations show that metabolic flux analysis can be used as a tool to gain insight into the metabolism of algae and ultimately can be used for the maximization of algal biomass and product yield.
Starting Page	253
Ending Page	266
Page Count	14
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	24
Issue Number	2
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-04-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Energy requirements Metabolic flux analysis (MFA) Chlamydomonas reinhardtii Maintenance Yield Respiration Freshwater & Marine Ecology Ecology Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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Metabolic modeling of Chlamydomonas reinhardtii: energy requirements for photoautotrophic growth and maintenance

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