NDLI: Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita

Content Provider	Springer Nature Link
Author	Xu, Jin Yin, Hengxia Wang, Wenying Mi, Qin Liu, Xiaojing
Copyright Year	2009
Abstract	The effects of sodium nitroprusside (SNP) on callus induction and shoot regeneration of Dioscorea opposite Thunb. have been studied. Application of 40 μM of SNP depresses accumulation of H2O2 in tuber explants of Dioscorea opposita markedly. Supplementation of 40 μM of SNP to the Murashige and Skoog medium with combinations of benzylaminopurine (3 mg dm−3) and naphthaleneacetic acid (0.5 mg dm−3) reduces the browning of explants and increases the frequency of callus induction from tuber explants significantly. The regeneration frequency of adventitious shoot shows a significant increase in the presence of SNP. Further analysis indicates that treatment with 40 μM of SNP results in significant decreases in catalase and peroxidase activity, while increasing the activity of superoxide dismutase. Supplementation with 40 μM of SNP also promotes the accumulation of non-enzymic antioxidants, including proline and glutathione. The effects on callus induction and shoot regeneration promoted by SNP were reversed by the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)- 4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide. These results indicate that the exogenously applied NO-donor SNP alleviates browning of tuber explants by reducing H2O2 accumulation, thereby promoting a higher in vitro proliferation frequency of D. opposita.
Starting Page	279
Ending Page	285
Page Count	7
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	59
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-10-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Micropropagation Sodium nitroprusside Browning Oxidative stress Agriculture Plant Physiology Plant Anatomy/Development Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dioscorea opposita Thunb. α-mannosidase belongs to the glycosyl hydrolase family 38

In vitro plant regeneration from organogenic callus of Curcuma kwangsiensis Lindl. (Zingiberaceae)

Effect of plant growth regulators on plant regeneration of Dioscorea remotiflora (Kunth) through nodal explants

The effect of carbon source on callus induction and regeneration ability in Pharbitis nil

Pyramid Screening: Combining Three Genetic Screens into One Efficient Screen for Shoot Regeneration Mutants in Arabidopsis thaliana

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata

Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.

Epigenetic instability in genetically stable micropropagated plants of Gardenia jasminoides Ellis

Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita

Similar Documents

Dioscorea opposita Thunb. α-mannosidase belongs to the glycosyl hydrolase family 38

In vitro plant regeneration from organogenic callus of Curcuma kwangsiensis Lindl. (Zingiberaceae)

Effect of plant growth regulators on plant regeneration of Dioscorea remotiflora (Kunth) through nodal explants

The effect of carbon source on callus induction and regeneration ability in Pharbitis nil

Pyramid Screening: Combining Three Genetic Screens into One Efficient Screen for Shoot Regeneration Mutants in Arabidopsis thaliana

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata

Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.

Epigenetic instability in genetically stable micropropagated plants of Gardenia jasminoides Ellis

Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita