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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shabannejad Mamaghani, Morteza Assareh, Mohammad Hassan Omidi, Mansoor Matinizadeh, Mohammad Ghamari Zare, Abbas Shahrzad, Shokofeh Forootan, Massih |
| Copyright Year | 2009 |
| Abstract | The green twigs of 1-year-old Eucalyptus microtheca F. Muell seedlings were cultured on modified MS medium, supplemented with α-naphthalene acetic acid (NAA) and kinetin (Kin) hormones at 12 different concentrations. After 4 weeks, the combination of 1 mg l−1 NAA + 1 mg l−1 Kin induced the highest number of axillary shoots. Meanwhile, embryogenic calli were observed in media containing 4 mg l−1 NAA + 0.5 mg l−1 Kin, without any regeneration. The hormone treatments were followed by subculturing the twigs in different levels of thidiazuron (TDZ). The combination of 1 mg l−1 NAA + 1 mg l−1 Kin together with 0.01 mg l−1 TDZ resulted in an increase of direct shoot, while higher amounts of TDZ led to adventitious shoot induction. Somatic embryogenesis was observed in the treatment containing 0.01 mg l−1 TDZ + 4 mg l−1 NAA + 0.5 mg l−1Kin. The peroxidase (POD) band patterns in regenerated plantlets were investigated in order to determine the effect of different levels of TDZ on loci synthesis. A dimer locus, a tetramer locus and two epigenetic bands (a new band for NAA + Kin and the other for TDZ) were observed in the POD profiles. In case of low (0.01 mg l−1 and 0.1 mg l−1) levels of TDZ, one heterozygote allele was disappeared from dimer locus, while at higher TDZ levels, the dimer locus lost its stability and tetramer locus showed a high activity. Thus, POD allele patterns seems to be a feasible marker for different types of regeneration. |
| Starting Page | 199 |
| Ending Page | 205 |
| Page Count | 7 |
| File Format | |
| ISSN | 01676903 |
| Journal | Plant Growth Regulation |
| Volume Number | 59 |
| Issue Number | 3 |
| e-ISSN | 15735087 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-08-19 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adventitious shoot Direct shoot Eucalyptus Peroxidase Somatic embryogenesis Thidiazuron Agriculture Plant Physiology Plant Anatomy/Development Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Plant Science Agronomy and Crop Science |
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