NDLI: Epigenetic instability in genetically stable micropropagated plants of Gardenia jasminoides Ellis

Content Provider	Springer Nature Link
Author	Wu, Ying Wu, Rui Zhang, Bangjiao Jiang, Tingting Li, Ning Qian, Kai Liu, Bao Zhang, Jian
Copyright Year	2011
Abstract	Gardenia jasminoides Ellis is an evergreen tropical plant and favorite to gardeners throughout the world. Several studies have documented that in vitro micropropagation can be used for clonal propagation of G. jasminoides Ellis, the efficiency remained low. In addition, no information is available on the genetic and epigenetic fidelity of the micropropagated plants. Here, we report on a simplified protocol for high efficient micropropagation of G. jasminoides Ellis cv. “Kinberly” based on enhanced branching of shoot-tips as explants. The protocol consisted of sequential use of three media, namely, bud-induction, elongation and root-induction. By using two molecular markers, amplified fragment length polymorphism (AFLP) and methylation sensitive amplified polymorphism (MSAP), we analyzed the genetic and DNA methylation pattern stability of 23 morphologically normal plants randomly taken from a sub-population (>100) of micropropagated plants originated from a single shoot-tip. We found that of >1,000 scored AFLP bands across the 23 micropropagated plants, no incident of genetic variation was detected. In contrast, of 750 scored MSAP bands, moderate but clear alteration in several DNA methylation patterns occurred in the majority of the 23 micropropagated plants. The changed methylation patterns involved both CG and CHG sites representing either hyper- or hypo-methylation, which occurred without altering the total methylation levels partly due to concomitant hyper- and hypo-methylation alterations. Our results indicated that epigenetic instability in the form of DNA methylation patterns can be susceptible to the in vitro micropropagation process for G. jasminoides Ellis, and needs to be taken into account in the process of large-scale commercial propagation of this plant.
Starting Page	137
Ending Page	143
Page Count	7
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	66
Issue Number	2
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Micropropagation Gardenia jasminoides Ellis Genetic stability DNA methylation Agriculture Plant Anatomy/Development Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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