NDLI: Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

Content Provider	Springer Nature Link
Author	Bairu, Michael W. vák, Ondřej Doležal, Karel Staden, Johannes
Copyright Year	2010
Abstract	Changes in cytokinin (CK) profiles and their physiological implications in micropropagated Harpagophytum procumbens [(Burch.) DC. ex Meisn.] tissues in relation to shoot-tip necrosis (STN) and CK treatments were studied. Total CK content was quantified in benzyladenine (BA)-treated necrotic and normal plantlets and in plantlets treated with the CKs BA, meta-topolin (mT) and meta-topolin riboside (mTR) with and without the auxin indole-3-acetic acid (IAA). Generally necrotic shoots yielded more total CK compared to normal shoots. Cytokinin accumulation was higher at the basal section (basal > middle > top). Further analysis of the CKs based on structural and functional forms revealed excessive accumulation of 9-glucosides (deactivation products—toxic metabolites) and limited amounts of O-glucosides (storage forms—re-utilizable) in necrotic and BA-treated shoots compared to normal and topolin-treated cultures. The addition of IAA enhanced the formation of 9-glucosides in BA-treated cultures but reduced it in topolin-treated cultures. The symptom of STN could therefore be attributed to conversion of active cytokinins to other forms such as 9-glucosides which are neither active nor reversibly sequestrated to active forms. Literature shows that metabolites like 9-glucosides of BA have a detrimental effect in plant tissue culture.
Starting Page	105
Ending Page	114
Page Count	10
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	63
Issue Number	2
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Auxin cytokinin interaction Cytokinin purification and analysis Devil’s claw Glucosylation Harpagophytum procumbens Micropropagation Pedaliaceae Shoot-tip necrosis Plant Anatomy/Development Plant Physiology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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