NDLI: A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development

Content Provider	Springer Nature Link
Author	Gómez Requeni, P. Conceição, L. E. C. Olderbakk Jordal, A. E. Rønnestad, I.
Copyright Year	2010
Abstract	Zebrafish is one of the most used vertebrate model organisms in molecular and developmental biology, recently gaining popularity also in medical research. However, very little work has been done to assess zebrafish as a model species in nutritional studies in aquaculture in order to utilize the methodological toolbox that this species represents. As a starting point to acquire some baseline data for further nutritional studies, growth of a population of zebrafish was followed for 15 weeks. Furthermore, whole body proteome was screened during development by means of bi-dimensional gel electrophoresis and mass spectrometry. Fish were reared under best practice laboratory conditions from hatching until 103 days post-fertilization (dpf) and regularly fed ad libitum with Artemia nauplii from 12 dpf. A growth burst occurred within 9–51 dpf, reaching a plateau after 65 dpf. Fork length and body weight were significantly lower in males than in females from 58 dpf onwards. Proteomics analysis showed 28 spot proteins differently expressed through development and according to sex. Of these proteins, 20 were successfully identified revealing proteins involved in energy production, muscle development, eye lens differentiation, and sexual maturation. In summary, zebrafish exhibited a rapid growth until approximately 50 dpf, when most individuals started to allocate part of the dietary energy intake for sexual maturation. However, proteomic analysis revealed that some individuals reached sexual maturity earlier and already from 30 dpf onwards. Thus, in order to design nutritional studies with zebrafish fed Artemia nauplii, it is recommended to select a period between 20 and 40 dpf, when fish allocate most of the ingested energy for non-reproductive growth purposes.
Starting Page	1199
Ending Page	1215
Page Count	17
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	36
Issue Number	4
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-04-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Growth curve Development Nutrition Proteomics Zebrafish Danio rerio Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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