NDLI: Organization and promoter analysis of the zebrafish (Danio rerio) chemokine gene (CXC-64) promoter

Content Provider	Springer Nature Link
Author	Chen, Li Chen Wu, Jen Leih Shiau, Chyuan Yuan Chen, Jyh Yih
Copyright Year	2009
Abstract	Zebrafish CXC-64, a chemokine representing a superfamily of chemotactic cytokines present in fish, is involved in recruitment, activation, and response to inflammatory stimulation. We cloned and sequenced the genomic DNA of the zebrafish CXC-64 chemokine; it was most similar to CXCL11 from humans and CXCL10 from a catfish. The zebrafish CXC-64 gene is approximately 4.0 kb long and has a four-exon, three-intron structure common to the human CXCL11 gene. However, the promoter region includes a typical TATA box and multi-transcription factor-binding sequences. To understand the roles of lipopolysaccharide (LPS), poly I:poly C, and tumor necrosis factor (TNF)-α in regulating zebrafish CXC-64 expression, serial deletions were made in the promoter region of this clone. Different fragments of the zebrafish CXC-64 5′-flanking region were transfected into RAW264.7 (mouse macrophage; Abelson murine leukemia virus transformed) and zfl (zebrafish liver) cells and then treated with 0, 10, 50, 100, and 200 ng/ml LPS, poly I:poly C, or TNF-α. The results showed that the promoter activity presented dose-dependent effects in LPS-treated RAW264.7 cells, TNF-α-treated RAW264.7 cells, and LPS-treated zfl cells. These results reveal that the zebrafish CXC-64 chemokine gene promoter region can be induced by LPS in both human and fish cell lines, which suggests that it plays an important role in regulating LPS.
Starting Page	511
Ending Page	521
Page Count	11
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	36
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-04-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zebrafish Chemokine Gene expression Functional analysis Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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