NDLI: Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcarifer

Content Provider	Springer Nature Link
Author	Mohd Yusof, N. Y. Monroig, O. Mohd Adnan, A. Wan, K. L. Tocher, D. R.
Copyright Year	2010
Abstract	Lates calcarifer, commonly known as the Asian sea bass or barramundi, is an interesting species that has great aquaculture potential in Asia including Malaysia and also Australia. We have investigated essential fatty acid metabolism in this species, focusing on the endogenous highly unsaturated fatty acid (HUFA) synthesis pathway using both biochemical and molecular biological approaches. Fatty acyl desaturase (Fad) and elongase (Elovl) cDNAs were cloned and functional characterization identified them as ∆6 Fad and Elovl5 elongase enzymes, respectively. The ∆6 Fad was equally active toward 18:3n-3 and 18:2n-6, and Elovl5 exhibited elongation activity for C18–20 and C20–22 elongation and a trace of C22–24 activity. The tissue profile of gene expression for ∆6 fad and elovl5 genes, showed brain to have the highest expression of both genes compared to all other tissues. The results of tissue fatty acid analysis showed that the brain contained more docosahexaenoic acid (DHA, 22:6n-3) than flesh, liver and intestine. The HUFA synthesis activity in isolated hepatocytes and enterocytes using [1-14C]18:3n-3 as substrate was very low with the only desaturated product detected being 18:4n-3. These findings indicate that L. calcarifer display an essential fatty acid pattern similar to other marine fish in that they appear unable to synthesize HUFA from C18 substrates. High expression of ∆6 fad and elovl5 genes in brain may indicate a role for these enzymes in maintaining high DHA levels in neural tissues through conversion of 20:5n-3.
Starting Page	827
Ending Page	843
Page Count	17
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	36
Issue Number	4
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-06-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fatty acid Metabolism Desaturase Elongase cDNA Functional characterization Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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