NDLI: Spectrofluorimetric study of the interaction of methyl-parathion with fish serum albumin

Content Provider	Springer Nature Link
Author	Silva, Dilson Cortez Moreira, Madelayne Cunha Bastos, Vera Lúcia Freire Cunha Bastos, Jayme Martins Cortez, Célia
Copyright Year	2009
Abstract	The interaction of methyl-parathion with the albumin of Piaractus mesopotamicus (Holmberg 1887) (= pacu), a fish species typical of Brazilian rivers, was studied and the results compared with known values for human and bovine albumin obtained in an earlier investigation. Methyl-parathion (O,O-dimethyl O-p-nitrophenyl phosphorothioate) is an organophosphorous pesticide still used in agriculture and fish farming in many countries. The fluorescence quenching technique with tryptophan as a natural probe was used to detect for the presence of methyl-parathion. Fluorescence can be mathematically expressed by the Stern–Volmer equation to calculate quenching constants, and changes in the behavior of Stern–Volmer curves at different temperatures indicate the nature of the mechanism causing the quenching. Our results indicate that methyl-parathion forms a complex with fish albumin. The estimated association constant is 9.73 × 103 (± 4.9 × 102) M−1 at 25°C.
Starting Page	427
Ending Page	433
Page Count	7
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	36
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fish albumin Fluorescence quenching Methyl-parathion Organophosphorous pesticides Piaractus mesopotamicus Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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