NDLI: In vitro induction of tetraploids in Phlox subulata L.

Content Provider	Springer Nature Link
Author	Zhang, Zhihong Dai, Hongyan Xiao, Min Liu, Xin
Copyright Year	2007
Abstract	Tetraploid plants of Phlox subulata L. were induced successfully by treating shoot tips in vitro with colchicine. Shoot tips excised from in vitro shoots were treated with four different concentrations of colchicine (0.005, 0.01, 0.02, 0.04%) in solid MS medium supplemented with 4.54 μM TDZ and 0.49 μM IBA for 10, 20 or 30 days, respectively. The survival rates of shoots tips were affected by the concentration of colchicine and the duration of treatment. High concentration and longer duration reduced survival of the shoot tips, but the effect of duration of colchicine was more than that of concentration. Tetraploid plants were obtained in all of the treatments, but the percentages of tetraploids varied among different treatments, from 25.0% to 75.0%. The most efficient condition for inducing tetraploids was to treat shoot tips with 0.005% colchicine for 20 days, with 30.0% survival rate of shoot tips and 6 tetraploid plants out of 10 plants examined. The rooted tetraploid plants were transplanted successfully in a solar greenhouse. Under the same growing condition, significant varieties in flower bud and flower sizes were detected between 2x and 4x plants. The flower diameters of tetraploid and diploid plants were 2.91 cm and 2.24 cm, respectively.
Starting Page	59
Ending Page	65
Page Count	7
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	159
Issue Number	1-2
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-05-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phlox subulata L. Tetraploid Induction Colchicine Shoot tip Flower diameter Ecology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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