NDLI: Cloning and characterization of a novel low molecular weight glutenin subunit gene at the Glu-A3 locus from wild emmer wheat (Triticum turgidum L. var. dicoccoides)

Content Provider	Springer Nature Link
Author	Li, Xiaohui Wang, Aili Xiao, Yinghua Yan, Yueming He, Zhonghu Appels, Rudi Ma, Wujun Hsam, S. L. K. Zeller, F. J.
Copyright Year	2007
Abstract	Amplification of the coding region, and upstream and downstream sequences of a low-molecular-weight glutenin subunits (LMW-GS) gene from wild emmer wheat (Triticum turgidum L. var. dicoccoides, 2n = 4x = 28, AABB) accession TD22 was carried out using designed allele-specific PCR (AS-PCR) primers. The complete 1,176 bp sequence of a novel LMW-i type subunit gene at the Glu-A3 locus, named LMW-TD22, is described. Analysis of the nucleotide and deduced amino acid sequences showed that this gene possessed striking characteristics although its molecular structure was generally similar to those of previously reported i-type LMW-GS genes that were isolated from common wheat and related species. The deduced amino acid sequence of LMW-TD22 gene contained 390 amino acid residues with the predicted molecular weight being 43,009.3 Da, which appeared to be the longest gene among the cloned LMW-i type genes from bread wheat and related species. The distinct feature of LMW-TD22 was two long polyglutamine stretches of 12 and 17 glutamines occurring in the repetitive and C-terminal domains as well as a cysteine residue present in the seventh amino acid residue of the signal peptide. These polyglutamine repeats are believed to improve the structure of gluten polymer and increase the strength of dough formed from the polymer. In addition, the putative 44 k subunit encoded by LMW-TD22 was verified by N-terminal microsequencing, gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis. Certain types of post-translational modifications, such as phosphorylation and glycosylation, may be associated with this LMW-i type subunit.
Starting Page	181
Ending Page	190
Page Count	10
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	159
Issue Number	1-2
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-07-17
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Wild emmer wheat LMW-i glutenin gene AS-PCR 2D PAGE Mass spectrometry Ecology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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