NDLI: In vitro induction of tetraploids in ornamental Ranunculus

Content Provider	Springer Nature Link
Author	Dhooghe, Emmy Denis, Sylvie Eeckhaut, Tom Reheul, Dirk Labeke, Marie Christine
Copyright Year	2009
Abstract	This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus ‘Alfa’ in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation. Trifluralin at a concentration of 2 μM resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine treatment of 200 μM, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration of 1 μM. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested (10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine (100 μM) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed, for trifluralin only a concentration of 10 μM affected viability whereas for oryzalin, concentration as well as exposure period were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells.
Starting Page	33
Ending Page	40
Page Count	8
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	168
Issue Number	1
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-01-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colchicine Oryzalin Polyploidisation Ranunculaceae Trifluralin Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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