NDLI: Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Content Provider	Springer Nature Link
Author	Chen, Chao Hou, Xilin Zhang, Hongxin Wang, Guilan Tian, Limin
Copyright Year	2011
Abstract	Tetraploids plants of Anthurium andraeanum “Arizona” were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7 h, and then were transferred into Murashige and Skoog medium containing 3 mg/l BAP + 0.2 mg/l 2,4-D. After 60 days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation.
Starting Page	137
Ending Page	145
Page Count	9
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	181
Issue Number	2
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anthurium andraeanum Tetraploid Colchicine Flow cytometry Variation Plant Genetics & Genomics Biotechnology Plant Physiology Plant Pathology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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