NDLI: Production of 2n pollen of Asiatic hybrid lilies by nitrous oxide treatment

Content Provider	Springer Nature Link
Author	Akutsu, Masako Kitamura, Satomi Toda, Ryo Miyajima, Ichiro Okazaki, Keiichi
Copyright Year	2006
Abstract	Tetraploid varieties of lilies have superior agronomic traits such as large flowers and resistance to physiological disorders. In the present study, we attempted to induce 2n pollen of Asiatic hybrid lilies by arresting the meiotic process with nitrous oxide (N2O) gas. To determine which meiotic stage is optimal for induction of 2n pollen, plants with attached buds at different meiotic stages were treated with N2O for 24 h in a pressure-tolerant cylinder. A few 2n pollen grains were induced using plants with anthers in prophase I, whereas mixed pollen grains of differing size were produced using plants undergoing meiotic metaphase predominantly in anthers. Although normal lily pollen grains are elliptical, nitrous oxide exposure induced giant pollen grains that appeared spherical. Flow cytometry analysis showed that the giant pollen grains were diploid. When mixed pollen that included normal and giant pollen was crossed to tetraploid cultivars, the resulting seedlings were tetraploid and aneuploid, indicating that the giant pollen grains were diploids that could generate tetraploid seedlings through fusion to diploid eggs supplied from a tetraploid female parent. Thus, treatment with N2O is useful for the production of 2n lily pollen and may provide a new approach for tetraploid lily breeding.
Starting Page	143
Ending Page	152
Page Count	10
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	155
Issue Number	1-2
e-ISSN	15735060
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-12-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chromosome doubling Flow cytometry Lilium Meiosis Nitrous oxide 2n pollen Ecology Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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