NDLI: Slow-growing pollen-tube of colchicine-induced 2n pollen responsible for low triploid production rate in Populus

Content Provider	Springer Nature Link
Author	Zhao, Chenguang Tian, Mengdi Li, Yanjie Zhang, Pingdong
Copyright Year	2017
Abstract	To reveal the mechanism of low triploid production, meiotic observation, induction of 2n pollen by colchicine, hybridization of colchicine-induced 2n pollen and pollen germination were conducted in Populus tomentosa clone 5088. Meiosis of the PMCs was initiated 24 h after it was cultured in the greenhouse. Results showed that the meiosis developed in a consecutive, asynchronous process. Analysis of the occurrence rate of colchicine-induced 2n pollen using the GLM-univariate revealed significant differences among the dominant meiotic stages (F = 45.822, P = 0.000). Significant differences also occurred among various colchicine injection times (F = 10.150, P = 0.004). The most effective stage for colchicine-induced 2n pollen occurred between the zygotene to diakinesis substages. Sixty-eight triploids were confirmed with both flow cytometric analysis and chromosome number-counting among the 3346 offspring. The highest triploid induction rate was 13.2%—significantly lower than the expected triploid production rate of 47.0%—indicating that the colchicine-induced 2n pollen was weak during fertilization. We found positive correlation between the efficiency of triploid production and the frequency of colchicine-induced 2n pollen (r = 0.961, P = 0.001) which suggests that the triploid production rate increased as the occurrence rate of colchicine-induced 2n pollen increased. Analysis using the GLM-univariate indicated that both pollen types (F = 87.50, P < 0.001) and durations of pollen germination (F = 7.61, P = 0.002) did have significant effects on the lengths of pollen-tubes. Haploid pollen-tubes were significantly longer than colchicine-induced 2n pollen-tubes. This suggests that haploid pollen has an advantage during fertilization.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	213
Issue Number	4
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-03-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Populus tomentosa Meiosis 2n pollen Pollen-tube Triploid production Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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