NDLI: Biotransformation of gypenoside XVII to compound K by a recombinant β-glucosidase

Content Provider	Springer Nature Link
Author	Zhong, Fei Liang Dong, Wei Wei Wu, Songquan Jiang, Jun Yang, Deok Chun Li, Donghao Quan, Lin Hu
Copyright Year	2016
Abstract	To study the β-glucosidase gene (bgy1) from Lactobacillus brevis that was cloned and expressed in Escherichia coli BL21 (DE3) and then using it for the biotransformation of gypenoside XVII.The bgy1 gene consists of 2283 bp encoding 761 amino acids, with homology to the glycosyl hydrolase family-3 protein domain. The enzyme (Bgy1) hydrolyzed the glucose moieties at the C-3 position and the outer glucose moieties at the C-20 position of gypenoside XVII. Using 0.1 mg enzyme ml−1 in 20 mM sodium phosphate buffer at 30 °C and pH 6.0, 1 mg gypenoside XVII ml−1 was transformed into 0.58 mg compound K ml−1 within 6 h, with a corresponding molar conversion yield of 89 %.The recombinant Bgy1 is considered potentially useful for the practical preparation of compound K.
Starting Page	1187
Ending Page	1193
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	7
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-04-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotransformation Compound K β-Glucosidase Gypenoside XVII Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Biotransformation of gypenoside XVII to compound K by a recombinant β-glucosidase