NDLI: Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng

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Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng

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Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng

Content Provider	Springer Nature Link
Author	Cai, Kexin Wang, Jiawen Wang, Min Zhang, Hui Wang, Siming Zhao, Yu
Copyright Year	2016
Abstract	To establish an efficient expression system for a fusion protein GST-pgLTP (Lipid Transfer Protein) and to test its antifungal activity.The nucleotide sequence of LTP gene was obtained from Panax ginseng using RT-PCR. The ORF of the cDNA is 363 bp, codING for a protein OF 120 amino acids with a calculated MW of 12.09 kDa. The pgLTP gene with a His6-tag at the C-terminus was cloned into the pGEX-6p1 vector to generate a GST-fusion pgLTP protein construct that was expressed in Escherichia coli Rosetta. Following purification by Ni–NTA, the fusion protein exhibited antifungal activity against five fungi found in ginseng.The fusion protein GST-pgLTP has activity against a broad spectrum of phytopathogenic fungi, and can potentially be adapted for production to combat fungal diseases that affect P. ginseng.
Starting Page	1229
Ending Page	1235
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	7
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-04-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antifungal activity Lipid transfer proteins Panax ginseng Protein expression and purification Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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