NDLI: Efficient kinetic resolution of secondary alcohols using an organic solvent-tolerant esterase in non-aqueous medium

Content Provider	Springer Nature Link
Author	Gao, Wenyuan Fan, Haiyang Chen, Lifeng Wang, Hualei Wei, Dongzhi
Copyright Year	2016
Abstract	To identify an esterase-mediated kinetic resolution of secondary alcohols in non-aqueous medium.An esterase, EST4, from a marine mud metagenomic library, showed high activity and enantioselectivity for the kinetic resolution of secondary alcohols in non-aqueous medium. Using 1-phenylethanol as the model alcohol, the effects of organic solvents, acyl donors, molar ratio, temperatures and biocatalyst loading on the kinetic resolution catalyzed by the EST4 whole-cell biocatalyst were investigated and optimized. The optimized methodology was effective on resolving 16 various racemic secondary alcohols in neat n-hexane, providing excellent enantiomeric excess (up to 99.9 % ee). Moreover, EST4 exhibited a strong tolerance for high substrate concentration (up to 1 M), and the optical purity of the desired secondary alcohols was kept above 99 % ee.The esterase EST4 is a promising biocatalyst for the enantioselective synthesis of various alcohols and esters with interesting practical applications.
Starting Page	1165
Ending Page	1171
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	7
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Enantioselectivity Esterase High substrate concentration tolerance Kinetic resolution alcohols Transesterification Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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