NDLI: Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients

Content Provider	Springer Nature Link
Author	Ostrowski, Maggie A. Huang, Eva Y. Surya, Vinay N. Poplawski, Charlotte Barakat, Joseph M. Lin, Gigi L. Fuller, Gerald G. Dunn, Alexander R.
Copyright Year	2015
Abstract	Endothelial cells (ECs) line the interior of blood and lymphatic vessels and experience spatially varying wall shear stress (WSS) as an intrinsic part of their physiological function. How ECs, and mammalian cells generally, sense spatially varying WSS remains poorly understood, due in part to a lack of convenient tools for exposing cells to spatially varying flow patterns. We built a multiplexed device, termed a 6-well impinging flow chamber, that imparts controlled WSS gradients to a six-well tissue culture plate. Using this device, we investigated the migratory response of lymphatic microvascular ECs, umbilical vein ECs, primary fibroblasts, and epithelial cells to WSS gradients on hours to days timescales. We observed that lymphatic microvascular ECs migrate upstream, against the direction of flow, a response that was unique among all the cells types investigated here. Time-lapse, live cell imaging revealed that the microtubule organizing center relocated to the upstream side of the nucleus in response to the applied WSS gradient. To further demonstrate the utility of our device, we screened for the involvement of canonical signaling pathways in mediating this upstream migratory response. These data highlight the importance of WSS magnitude and WSS spatial gradients in dictating the cellular response to fluid flow.
Starting Page	2261
Ending Page	2272
Page Count	12
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	44
Issue Number	7
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2015-11-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mechanotransduction Cell migration Vascular biology Fluid mechanics Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fluid Flow Mechanotransduction in Vascular Smooth Muscle Cells and Fibroblasts

The Effect of Noisy Flow on Endothelial Cell Mechanotransduction: A Computational Study

Shear Stress Variation Induced by Red Blood Cell Motion in Microvessel

Mechanical Response of a Calcified Plaque Model to Fluid Shear Force

Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways

High Fluid Shear Stress and Spatial Shear Stress Gradients Affect Endothelial Proliferation, Survival, and Alignment

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Effects of Disturbed Flow on Endothelial Cells

Cellular Fluid Mechanics and Mechanotransduction

Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients

Similar Documents

Fluid Flow Mechanotransduction in Vascular Smooth Muscle Cells and Fibroblasts

The Effect of Noisy Flow on Endothelial Cell Mechanotransduction: A Computational Study

Shear Stress Variation Induced by Red Blood Cell Motion in Microvessel

Mechanical Response of a Calcified Plaque Model to Fluid Shear Force

Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways

High Fluid Shear Stress and Spatial Shear Stress Gradients Affect Endothelial Proliferation, Survival, and Alignment

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Effects of Disturbed Flow on Endothelial Cells

Cellular Fluid Mechanics and Mechanotransduction

Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients