NDLI: Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Content Provider	Springer Nature Link
Author	Isenberg, Brett C. Williams, Chrysanthi Tranquillo, Robert T.
Copyright Year	2006
Abstract	It is generally accepted that endothelialization and subsequent development of a functional endothelium are of paramount importance to the success of any bioartificial artery. In this study, we aimed to assess the ability of smooth muscle cell-remodeled, fibrin-based media-equivalents (MEs) to be endothelialized, examine the morphological changes of endothelial cells (ECs) associated with exposure to physiologically-relevant shear stress in a custom-built bioreactor, and determine if adherent ECs are capable of withstanding average physiological shear stresses. It was found that MEs could be readily endothelialized with surface coverages of 98.8 ± 0.9% after two days, and the ECs expressed von Willebrand factor. Furthermore, EC retention remained high (steady: 96.5 ± 4.4%, pulsatile: 94.3 ± 4.3%) under exposure to physiologically relevant shear stresses for 48 h. The results indicate that these MEs are conducive to generating an EC monolayer, with the ECs possessing adhesion strength sufficient to withstand physiological shear stress and maintain a normal phenotype.
Starting Page	971
Ending Page	985
Page Count	15
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	34
Issue Number	6
e-ISSN	15739686
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-05-24
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tissue engineering Fibrin gel Shear stress Fluid flow Smooth muscle cell Endothelial cell Vascular graft Biomedical Engineering Biophysics/Biomedical Physics Mechanics Biochemistry Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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