NDLI: Microfluidic platform for human placenta-derived multipotent stem cells culture and applied for enhanced neuronal differentiation

Content Provider	Springer Nature Link
Author	Cheng, Yu Che Tsao, Chia Wen Chiang, Meng Zhi Chung, Chih Ang Chien, Chih Cheng Hu, Wei Wen Ruaan, Ruoh Chyu Li, Chuan
Copyright Year	2014
Abstract	Stem cell research grows rapidly in recent years due to its potential in clinical applications. Since the stem cell behavior is influenced by environmental cues, investigation of the stem cell differentiation under chemical as well as physical stimulation environment is important. Under traditional petri dish culturing conditions, directly applying physical stimulation on the cells is difficult. Microfluidic chip, on the other hand, provides a manageable platform to stimulate cells. In this research, we use microfluidic chip to culture human placenta-derived multipotent stem cells (PDMCs) and apply chemical and physical stimulations to the cells. PDMCs is a newly developed human stem cell source that are easier to access with less ethical issues compare to other human stem cells. This research shows that the microfluidic platform could provide adequate in vitro microenvironment that maintain pluripotency and proliferative potential for PDMCs culture. PDMCs were successfully differentiate to neuronal cells through chemical and physical–chemical stimulation on the microfluidic platform. Our results show that through physical shear stress stimulation, PDMCs can result in enhanced neuronal cell differentiation.
Starting Page	587
Ending Page	598
Page Count	12
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	18
Issue Number	4
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-07-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidic Cell culture Placenta-derived multipotent stem cells Physical stimulation Chemical stimulation Neuronal cell differentiation Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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