NDLI: Spiral-shaped inertial stem cell device for high-throughput enrichment of iPSC-derived neural stem cells

Content Provider	Springer Nature Link
Author	Song, Hongjun Rosa, Jenna M. Wang, Yi Garson, Charles J. Prabhakarpandian, Balabhaskar Pant, Kapil Klarmann, George J. Alvarez, Luis M. Lai, Eva
Copyright Year	2017
Abstract	Stem cell enrichment plays a critical role in both research and clinical applications. The typical method for stem cell enrichment may use invasive processes and takes a long period of time. Spiral-shaped microfluidic devices, which combine lift and Dean drag forces to direct cells of different sizes into separate trajectories, can be used to noninvasively process samples at a rate of milliliters per minute. This paper presents a simple 2-loop spiral-shaped inertial microfluidic devices with the aid of sheath flow to enrich neural stem cells (NSCs), derived from induced pluripotent stem cells. NSCs and spontaneously differentiated non-neural cells were mixed and flowed through the spiral-shaped devices. Samples collected at the outlets were analyzed for purity and recovery. It was found that the device focused the NSCs into a narrow trajectory, which could then be collected in two out of the eight outlets. The device was tested at different flow rates and found that the most highly enriched fractions (2.1×) with NSCs recovery 93% were achieved at the flow rate (3 ml/min). Next, we extended our investigation from 2-loop design to 10-loop design to eliminate the use of sheath flow. NSCs were enriched to 2.5×, but only 38% of the NSCs were recovered from the most enriched fractions. Spiral-shaped microfluidic devices are capable of rapid, label-free enrichment of target stem cells, and have great potential in point-of-care tissue preparation.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	21
Issue Number	4
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-03-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Induced pluripotent stem cells Neural stem cell Inertial microfluidic Enrichment Purification Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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