NDLI: Three-dimensional microfluidic chip for the extraction of mitochondrial DNA

Content Provider	Springer Nature Link
Author	Chang, Chen Min Chiou, Li Fang Lin, Chun Che Shieh, Dar Bin Lee, Gwo Bin
Copyright Year	2010
Abstract	This study reports an integrated microfluidic chip manufactured by micro-electro-mechanical-system (MEMS) technology used for the automatic extraction of mitochondrial deoxyribonucleic acid (mtDNA). Mitochondria is a key organelle associated with aging, degenerative diseases, and cancers. An alteration in mtDNA may be induced by oxidative stress in tissues and cells and may be used as an indicator for diseases associated with aging. Hence, there is a great need to establish a quantitative and qualitative measurement method to evaluate dysfunction in mitochondria. Traditional processes for the measurement of alterations in mtDNA involves micro-cloning, direct sequencing, real-time polymerase chain reaction (PCR) processing, and microarray detection; all of which are time-consuming and labor-intensive processes. Especially, the extraction of mtDNA from cell samples that require precision and a lengthy process. This microfluidic chip integrates micropumps, a micromixer and a micro temperature module in a three-dimensional structure to automate the entire process of the extraction of the mtDNA. Compared with its conventional counterparts, the advantages of this microchip include less sample and reagent consumption, higher extraction performance, a shorter analysis time, and automation. The developed microsystem is promising for the extraction of mtDNA and can be applied in further mitochondrial studies.
Starting Page	489
Ending Page	498
Page Count	10
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	9
Issue Number	2-3
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-01-14
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mitochondrial DNA Microfluidics Rapid extraction Nanofluidics High efficiency Microchip Biomedical Engineering Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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