NDLI: Selective DNA extraction with microparticles in segmented flow

Content Provider	Springer Nature Link
Author	Verbruggen, Bert Leirs, Karen Puers, Robert Lammertyn, Jeroen
Copyright Year	2014
Abstract	Droplet-based segmented flow microfluidic systems have proven their potential for high throughput detection and quantification of analytes. However, the required sample preparations are often performed off-chip, as on-chip methods are lacking. Microparticles are especially suited for the extraction and purification of target molecules from a sample and are successfully used in other microfluidic systems and in laboratory-scale methods. The current magnetic separation methods in segmented flow microfluidics are limited in their function to purify, as only a limited part of the original droplet volume can be removed from the particles. In this paper, we report the implementation of a selective DNA extraction assay with microparticles in a segmented flow microfluidic system. The combination of magnetic separation with asymmetric droplet splitting allows the removal of 90 % or even 95 % of the original sample volume in a single separation step. It is shown that the hybridization and capture efficiency of the particles are identical for off-chip and on-chip methods. Next, the effect of the particle separation efficiency on the extraction efficiency is tested for different splitting regimes. When up to 90 % of the droplet volume is removed, nearly all particles are correctly separated and the non-separated particle fraction remains below 5 %. Only if 95 % of the original volume is removed, the unseparated fraction becomes significant (>10 %). Finally, the impact of separation at a higher splitting ratio for the repeated washing of the particles is discussed. With this novel system, more complex and relevant bioassays can be implemented completely in a droplet-based segmented flow context.
Starting Page	293
Ending Page	303
Page Count	11
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	18
Issue Number	2
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-06-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Magnetic microparticles DNA extraction Droplet-based segmented flow Segmented flow microfluidics On-chip magnetic separation Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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