NDLI: A simple method of constructing microfluidic solid-state quantum dot molecular beacon array for label-free DNA detection

Content Provider	Springer Nature Link
Author	Liu, Hongwei Shu, Weiliang Liu, Zongbin Zhang, Baoyue Feng, Hongtao Chen, Yan
Copyright Year	2017
Abstract	Solid-state molecular beacons show great potential for label-free biomarker detection, however, it is challenging to construct robust and homogenous quantum dot molecular beacon microarrays in a microfluidic platform. Here, we report a simple and cost-effective method of constructing a mercaptopropionic acid quantum dot (MPA-QD) microarray in a microfluidic platform using a PDMS through-hole structure. This method combines soft lithography and surface functionalization to achieve uniform QD immobilization in a microfluidic network. With the simple fabricated quantum dot microarray sensor integrated in the microfluidic device, label-free biomarker detection was performed with high efficiency, specificity and sensitivity. We performed cardiovascular biomarker detection, and our microfluidic QD molecular beacon platform achieved target DNA sequence identification at a low concentration of 1 nM/L.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	21
Issue Number	4
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-03-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Solid-state molecular beacon Quantum dot Microfluidics Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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