NDLI: HPLC–DAD Analysis of Hydrochlorothiazide and Irbesartan in Hypertensive Patients on Fixed-Dose Combination Therapy

Content Provider	Springer Nature Link
Author	Coudoré, François Harvard, Laurent Lefeuvre, Sandrine Billaud, Eliane M. Beaune, Philippe Bobrie, Guillaume Azizi, Michel Progn, Patrice Laurent, Stéphane
Copyright Year	2011
Abstract	Hydrochlorothiazide (HCTZ) and the angiotensin II type 1 receptor antagonist (ARB) irbesartan (IRBE) are well-known antihypertensive drugs, frequently administered as a low-dose combination in a single pill. In this work, a simple, sensitive, and selective high-performance liquid chromatographic (HPLC) method with diode-array detection was developed for simultaneous determination of HCTZ and IRBE levels in the plasma of hypertensive patients given a fixed combination of 12.5 mg HCTZ and 300 mg IRBE. Compounds were extracted from acidified plasma samples with 3 mL ethyl acetate, and eluted at 6 and 19 min from a C4 column by elution with an acetonitrile–phosphate buffer (pH 3.6) mobile-phase gradient at a flow rate of 1 mL min−1. The assay was linear over the ranges 2.5–500 and 20–4,000 ng mL−1 for HCTZ and IRBE, respectively. Overall intra-assay and inter-assay variation were within acceptance limits. Limits of quantification were 2.5 and 20 ng mL−1 for HCTZ and IRBE, respectively. Plasma samples remained stable for 12 h at room temperature, through three thaw–freeze cycles, and for 2 and 7 months at −20 °C. In hypertensive patients, residual concentrations were 22.3 ± 6.0 and 241.8 ± 39.0 ng mL−1 for HCTZ and IRBE, respectively. There was no interference from other co-administered drugs. Despite the different physicochemical properties of HCTZ and IRBE, our method enables accurate measurement of both drugs for assessment of compliance by patients treated by fixed-dose combination therapy with HCTZ–IRBE.
Starting Page	559
Ending Page	565
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	74
Issue Number	7-8
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HPLC–DAD Hydrochlorothiazide Irbesartan Human plasma Pharmacy Organic Chemistry Proteomics Analytical Chemistry Plant Sciences Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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