NDLI: Monoamine Oxidase Inhibitor Screening by Proteoliposome Capillary Electrophoresis

Content Provider	Springer Nature Link
Author	Li, Bing Lv, Xuefei Qing, Hong Geng, Lina Deng, Yulin
Copyright Year	2011
Abstract	In this study, a new strategy for screening of enzyme inhibitors based on the interaction between enzyme and substrate was established. The bioactive proteoliposome reconstituted by monoamine oxidase (MAO) and liposome was added in the running buffers of capillary electrophoresis (CE) as pseudostationary phase to simulate the interaction between MAO and its substrate kynuramine (Kyn). The results showed that the relative migration time ratio (RMTR) of Kyn decreased obviously with increase of the proteoliposome, indicating strong interaction between proteoliposome and Kyn. After adding MAO inhibitors into the running buffers containing proteoliposome, the decline of the RMTR value of Kyn became slower. This occurred because MAO was inhibited to some degree by the inhibitors, weakening the interaction between MAO and Kyn. However, adding compounds which do not inhibit MAO activity into the running buffers did not affect the RMTR of Kyn obviously. The results obtained demonstrate that the method developed could characterize the inhibition of MAO inhibitors. Compared with traditional inhibitor screening methods, it does not need incubation outside the column to complete the enzyme catalytic reaction, so analysis is faster and less sample is consumed. With further development, the method might be chosen as a useful tool for screening of membrane protein inhibitors and identification of proteins associated with various diseases.
Starting Page	619
Ending Page	624
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	74
Issue Number	7-8
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis Proteoliposome Kynuramine Monoamine oxidase Inhibitor Pharmacy Organic Chemistry Proteomics Analytical Chemistry Plant Sciences Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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