NDLI: LC–MS–MS Method for Quantification of Hydralazine in BALB/C Mouse Plasma and Brain: Application to Pharmacokinetic Study

Content Provider	Springer Nature Link
Author	Liu, Yanying Li, Hui Luo, Hongjun Lin, Zhexuan Luo, Wenhong
Copyright Year	2011
Abstract	A rapid, sensitive and accurate high performance liquid chromatography method using tandem mass spectrometry detection for hydralazine in BALB/C mouse plasma and brain was developed and validated. The method involved a derivatization with 2,4-pentanedione at 50 °C for 1 h, and a step of solid phase extraction to purify and concentrate hydralazine derivative. Chromatographic separation was carried out on an Agilent ZORBAX SB-C18 column by elution with methanol–0.01 mol L−1 ammonium acetate (60:40, v/v). The multiple reaction monitoring transition used for quantification was m/z 225.2 → 129.5 in the electrospray positive ionization mode. Good linearity was obtained over the concentration range of 10–200 ng mL−1. The limits of detection were 0.49 and 1.05 ng mL−1 for hydralazine in mouse plasma and brain, respectively. The limits of quantitation were 1.5 and 3.18 ng mL−1 for hydralazine in mouse plasma and brain, respectively. Sample analysis time was 6 min including sample separation. The method was successfully applied to a pharmacokinetic study following intraperitoneal injection of hydralazine in BALB/C mice at the dose of 20 mg kg−1.
Starting Page	1183
Ending Page	1188
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	73
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Liquid chromatography-tandem mass spectrometry Pharmacokinetics Hydralazine Analytical Chemistry Biochemistry Plant Sciences Proteomics Pharmacy Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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