NDLI: An LC–ESI–MS–MS Method for Quantification of Tenofovir Octadecyloxyethyl Ester in Rat Plasma and Its Application to a Pharmacokinetic Study

Content Provider	Springer Nature Link
Author	Liu, Meng si Guo, Ji fen Yi, Hong Li, Hua Li, Zhuo rong Zhao, Chun jie
Copyright Year	2011
Abstract	Tenofovir octadecyloxyethyl ester (TOE) is a novel ester prodrug of tenofovir. It is currently developed as a promising antiviral preclinical candidate. A simple and sensitive liquid chromatography–tandem mass spectrometry (LC–MS–MS) method has been developed and validated to quantify TOE in rat plasma. The method involves a simple extraction of TOE and loratadine (internal standard) from rat plasma after protein precipitation. Chromatographic separation was performed on a C18 column by isocratic elution with acetonitrile and 5 mM ammonium acetate containing 0.03% ammonium hydroxide (70:30, v/v), at a flow rate of 0.3 mL min−1. MS detection was performed on an API 3000 triple quadrupole mass spectrometer operating in ESI positive ionization mode. Ions monitored in the multiple selected reaction monitoring mode were m/z 584→270 for TOE and m/z 383→337 for loratadine, respectively. A linear calibration curve was established over the concentration range from 12.5 to 2,500 ng mL−1 (r 2 = 0.9961) with the lower quantitative limit of 12.5 ng mL−1. The inter- and intra-day RSD were all <9.61%, while the accuracy (RE) was within the range from −8.40 to 4.20%. This novel method has been successfully applied to a pharmacokinetic study to obtain the oral concentration–time profile of TOE in rats.
Starting Page	51
Ending Page	57
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	74
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-05-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography–tandem mass spectrometry Tenofovir octadecyloxyethyl ester Tenofovir Pharmacokinetics Biochemistry Organic Chemistry Analytical Chemistry Plant Sciences Proteomics Pharmacy
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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