NDLI: Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS

Content Provider	Springer Nature Link
Author	Su, Meng xiang Di, Bin Hang, Tai jun Wang, Jun Yang, Da song Wang, Tai hu Meng, Rui
Copyright Year	2011
Abstract	A rapid, sensitive and highly selective liquid chromatography/tandem mass spectrometry (LC–MS–MS) using an atmospheric pressure chemical ionization (APCI) method was developed to determine the concentration of trans-resveratrol in rat plasma. Samples were extracted using ethyl acetate after daidzein was added as an internal standard (IS). The main advantages of this method were the high sensitivity (a lower limit of quantification, LLOQ, of 0.1 ng mL−1) and the short analysis time (total run time 3.2 min per sample). Separation was performed on a ZORBAX ODS column with a mobile phase that consisted of acetonitrile/water (48:52, v/v). trans-Resveratrol and IS were detected using a triple-quadrupole mass spectrometer in the selective reaction monitoring (SRM) mode with the parent-to-product quantifier transitions [M–H]− m/z 226.8 → 142.9 and [M–H]− m/z 252.9 → 252.9 for trans-resveratrol and IS, respectively. The method was confirmed to be accurate and precise with a linearity range of 0.1–500 ng mL−1 with r > 0.99. Recoveries for trans-resveratrol and IS were within 90–101%. The accuracy and precision for the assay were determined by calculating the intra- and inter-batch variation for quality control (QC) samples at four concentration levels with a relative standard deviation (RSD) of <15%. This method was successfully applied to determine trans-resveratrol in rat plasma and proved suitable for pharmacokinetic study after intragastric administration of trans-resveratrol in sustained release granules and normal granules, respectively.
Starting Page	1203
Ending Page	1210
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	73
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-04-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Mass spectrometry trans-Resveratrol Rat plasma Analytical Chemistry Biochemistry Plant Sciences Proteomics Pharmacy Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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