NDLI: LC–MS–MS Quantitative Determination of Brivudine in Human Plasma and Its Application to Pharmacokinetic Studies

Content Provider	Springer Nature Link
Author	Peng, Xiang Dong Tan, Zhi Rong Wu, Hui Zi Zhou, Gan Guo, Chen Xian Pei, Qi Cao, Shan Meng, Xiang Guang Wang, Yi Cheng Chen, Yao Guo, Dong Fan, Lan Zhang, Wei Zhou, Hong Hao
Copyright Year	2011
Abstract	A sensitive and specific liquid chromatography–electrospray ionization–tandem mass spectrometry method has been developed and validated for the identification and quantification of brivudine in human plasma using diclofenac as an internal standard. The method involves extraction with ethyl acetate. The analyte was separated on a C18 column and analyzed in multiple reaction monitoring mode with a negative electrospray ionization interface using the [M–H]− ions, m/z 332.8→m/z 80.9 for brivudine, m/z 293.6→m/z 249.5 for diclofenac. The method was validated over the concentration range of 5.54–2,836 μg L−1 for brivudine. The intra-and inter-day precisions were less than 8.91% in terms of relative standard deviation (RSD), and the accuracy was within −4.22% in terms of relative error (RE). The lower limit of quantification (LLOQ) was 5.54 μg L−1 with acceptable precision and accuracy. There were almost no matrix effects. Recovery of brivudine spiked in drug-free plasma was higher than 77.17%. The method was used to study the pharmacokinetic profile of brivudine in human plasma after oral administration of brivudine tablets.
Starting Page	1089
Ending Page	1095
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	73
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography LC–MS–MS Electrospray ionization Pharmacokinetic study Brivudine Analytical Chemistry Biochemistry Plant Sciences Proteomics Pharmacy Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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