NDLI: Optimization of LC/MS (APCI)+ Methods for the Determination of Possible Lutein Oxidation Products in Plasma and Tissues of Adult Rats

Content Provider	Springer Nature Link
Author	Sowmya, Poorigali Raghavendra Arathi, Bangalore Prabhashankar Vijay, Kariyappa Baskaran, Vallikannan Lakshminarayana, Rangaswamy
Copyright Year	2014
Abstract	In spite of lutein and its isomer zeaxanthin being richly available in natural sources, the role of these components on reduction of age-related macular degeneration, cancer, and cardiovascular disorders suggested that an update of the analytical procedure is required to determine the oxidative products and to understand their nutritional significance. In the present study, we have standardized and developed an improved method to obtain characteristic ions of lutein, zeaxanthin, and its major oxidative products in vivo (rats) using LC–MS (APCI)+. In addition, lutein and zeaxanthin isomer were separated on a C30 column with shorter run time with high resolution and calibrated on the basis of picomolar concentration on HPLC (DAD), with the lower detection limit of 0.125 for lutein and 0.128 pmol for zeaxanthin. Characteristic mass spectral ion for lutein is m/z 568.7 [M]+ and 551.5 [M + H–H2O]+ and for zeaxanthin isomer is m/z 568.8 [M]+, 569.8 [M + H]+. Further, optimized conditions produced structurally characteristic fragmented ions under standardized MS (APCI)+ conditions. Total ionic chromatogram together with fine UV–Visible and mass spectra were used to differentiate lutein isomers and its oxidative products, such as 523 [M+ + H+–3CH3], 479 [M+ + H+–6CH3], 551 [M+ + H+–H2O], 276.43 [M+–C22H19O], di-epoxides and 3′-oxolutein. The APCI mass spectral characteristics of major oxidative products of lutein in adult rat tissues are reported here for the first time, to our knowledge. These findings could provide new insights into lutein bioavailability and bioconversions with respect to health benefits.
Starting Page	1633
Ending Page	1642
Page Count	10
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	77
Issue Number	23-24
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Liquid chromatography–mass spectrometry Lutein Zeaxanthin Rat tissues Lutein oxidation products Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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