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| Content Provider | Springer Nature Link |
|---|---|
| Author | Bayrak, Ziya Kurbaglu, Sevinc Savaser, Ayhan Ozkan, Sibel A. Altunay, Hikmet Tas, Cetin Ozkan, Cansel Kose Ozkan, Yalcin |
| Copyright Year | 2013 |
| Abstract | To support real biological sample application, a simple, selective and rapid LC–MS method has been developed and validated for the sensitive determination of metoclopramide in rabbit blood, ex vivo permeation studies and pharmaceutical dosage form. LC–MS analysis was performed isocratically on a Zorbax SB-C18 column with a mobile phase consisting of methanol:ammonium acetate buffer (pH 3.0) 75:25 (v/v) at a flow rate of 0.70 mL min−1. The outlet of the column was connected to a single quadrupole mass spectrometer with positive mass spectrometric detection. Ions were detected in the positive multiple reaction monitoring mode. The assay was linear over the concentration range of 1.25–200 pg μL−1 with a limit of detection of 0.077 pg μL−1 for standard solutions and 2.5–200 pg μL−1 with a limit of detection of 0.42 pg μL−1 for serum samples. The method is applicable, covering a variety of pharmaceutical and biological studies. Metoclopramide was extracted from rabbit blood by liquid–liquid extraction using ether as the extraction solvent. The reproducibility of the method was found to be between 0.96 and 1.98 % (RSD) values. The proposed method has been extensively validated for the determination of metoclopramide in all working media. The sample preparations, flow rate and run time of the analytical systems are not time consuming. Moreover, for the stability of metoclopramide, the effect of temperature, UV light, H2O2, HCl and NaOH were also investigated. |
| Starting Page | 99 |
| Ending Page | 107 |
| Page Count | 9 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 77 |
| Issue Number | 1-2 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-10-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography–mass spectrometry Stability Validation Metoclopramide Chromatography Proteomics Pharmacy Laboratory Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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