NDLI: Preparative Purification of Epigallocatechin-3-gallate (EGCG) from Tea Polyphenols by Adsorption Column Chromatography

Content Provider	Springer Nature Link
Author	Wang, Weitao Ma, Chaoyang Chen, Shangwei Zhu, Song Lou, Zaixiang Wang, Hongxin
Copyright Year	2014
Abstract	In order to separate epigallocatechin-3-gallate (EGCG) from tea polyphenols, the adsorption ability of resins chosen toward catechins was investigated. According to static adsorption results of resins selected, polyamide displayed better selective adsorption ability between esterified and non-esterified catechins, and LSA-10 distinguished EGCG from ECG very well among esterified catechins. Therefore, columns packed with polyamide and resin LSA-10 were adopted in the first and second purification of EGCG, respectively. After the first-run purification, the product can be obtained with a 70.27 % EGCG purity and 80.21 % recovery. After the second-run separation, product contained an EGCG purity of 95.14 % and a total recovery of 76.78 % can be achieved. Moreover, a higher per volume adsorption material load amount 51.02 mg mL−1 tea polyphenols and yield 24.11 mg mL−1 EGCG were obtained in the study. Furthermore, only ethanol solution was used as elution reagent in the process. In the study, a method that is environmentally friendly, with lower cost and better repeatability has been developed. This result will be of help in development of tea resources and applications on a large scale.
Starting Page	1643
Ending Page	1652
Page Count	10
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	77
Issue Number	23-24
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-09-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adsorption column chromatography Catechin Epigallocatechin-3-gallate (EGCG) Purification Tea polyphenols Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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