NDLI: Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation

Content Provider	Springer Nature Link
Author	Alves, Eliana Carvalho, Carla M. B. Tomé, João P. C. Fausti, Maria A. F. Neves, Maria G. P. M. S. Tomé, Augusto C. Cavaleiro, José A. S. Cunha, Ângela Mendo, Sónia Almeida, Adelaide
Copyright Year	2008
Abstract	A faster and simpler method to monitor the photoinactivation process of Escherichia coli involving the use of recombinant bioluminescent bacteria is described here. Escherichia coli cells were transformed with luxCDABE genes from the marine bioluminescent bacterium Vibrio fischeri and the recombinant bioluminescent indicator strain was used to assess, in real time, the effect of three cationic meso-substituted porphyrin derivatives on their metabolic activity, under artificial (40 W m−2) and solar irradiation (≈620 W m−2). The photoinactivation of bioluminescent E. coli is effective (>4 log bioluminescence decrease) with the three porphyrins used, the tricationic porphyrin Tri-Py+-Me-PF being the most efficient compound. The photoinactivation process is efficient both with solar and artificial light, for the three porphyrins tested. The results show that bioluminescence analysis is an efficient and sensitive approach being, in addition, more affordable, faster, cheaper and much less laborious than conventional methods. This approach can be used as a screening method for bacterial photoinactivation studies in vitro and also for the monitoring of the efficiency of novel photosensitizer molecules. As far as we know, this is the first study involving the use of bioluminescent bacteria to monitor the antibacterial activity of porphyrins under environmental conditions.
Starting Page	1447
Ending Page	1454
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	35
Issue Number	11
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-08-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cationic porphyrins Photodynamic antimicrobial therapy Bioluminescence Escherichia coli Solar irradiation Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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