NDLI: Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering

Content Provider	Springer Nature Link
Author	Waegeman, Hendrik Soetaert, Wim
Copyright Year	2011
Abstract	Different hosts have been used for recombinant protein production, ranging from simple bacteria, such as Escherichia coli and Bacillus subtilis, to more advanced eukaryotes as Saccharomyces cerevisiae and Pichia pastoris, to very complex insect and animal cells. All have their advantages and drawbacks and not one seems to be the perfect host for all purposes. In this review we compare the characteristics of all hosts used in commercial applications of recombinant protein production, both in the area of biopharmaceuticals and industrial enzymes. Although the bacterium E. coli remains a very often used organism, several drawbacks limit its possibility to be the first-choice host. Furthermore, we show what E. coli strains are typically used in high cell density cultivations and compare their genetic and physiological differences. In addition, we summarize the research efforts that have been done to improve yields of heterologous protein in E. coli, to reduce acetate formation, to secrete the recombinant protein into the periplasm or extracellular milieu, and to perform post-translational modifications. We conclude that great progress has been made in the incorporation of eukaryotic features into E. coli, which might allow the bacterium to regain its first-choice status, on the condition that these research efforts continue to gain momentum.
Starting Page	1891
Ending Page	1910
Page Count	20
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	38
Issue Number	12
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Heterologous protein Recombinant protein production Escherichia coli Engineering Biopharmaceutical Industrial enzyme Biotechnology Inorganic Chemistry Biochemistry Microbiology Bioinformatics Genetic Engineering
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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