NDLI: Soluble expression of recombinant human CD137 ligand in Escherichia coli by co-expression of chaperones

Content Provider	Springer Nature Link
Author	Wang, Shuzhen Tan, Aimin Lv, Junfang Wang, Peng Yin, Xiaojin Chen, Yijun
Copyright Year	2011
Abstract	CD137 ligand (CD137L) is a member of the tumor-necrosis factor superfamily that binds CD137 to provide positive co-stimulatory signals for T cells activation. Co-stimulation through CD137/CD137L has become one of the promising approaches for cancer therapy. Previous reports have shown that CD137L expressed in Escherichia coli resulted in inclusion bodies or low yield. In this study, the effects of five different chaperone teams on the soluble expression of recombinant human CD137L protein were explored and analyzed. The poor expression of CD137L in the cytoplasm of E. coli was improved significantly by co-expression of chaperone GroES-GroEL-Tf. After dual induction and affinity chromatography, purified recombinant CD137L was obtained at a yield of 3 mg protein per liter with purity greater than 98% from original undetectable level. Additionally, the purified recombinant CD137L could bind CD137-positive cells in a dose-dependent manner, markedly promote the growth of activated mice T cells, and elevate the release of IL-2. The present work provides an effective system for soluble expression of functional human co-stimulatory molecule CD137L, which will facilitate the clinical developments of recombinant protein drugs.
Starting Page	471
Ending Page	476
Page Count	6
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	39
Issue Number	3
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-10-15
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Soluble expression Co-stimulatory molecule CD137L Escherichia coli Chaperones Inorganic Chemistry Biotechnology Microbiology Bioinformatics Genetic Engineering Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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