NDLI: Immobilization of the recombinant invertase INVB from Zymomonas mobilis on Nylon-6

Content Provider	Springer Nature Link
Author	Vallejo Becerra, Vanessa Vásquez Bahena, Jazmín Magdalena Santiago Hernández, José Alejandro Hidalgo Lara, María Eugenia
Copyright Year	2008
Abstract	The recombinant invertase INVB (re-INVB) from Zymomonas mobilis was immobilized on microbeads of Nylon-6, by means of covalent bonding. The enzyme was strongly and successfully bound to the support. The activity of the free and immobilized enzyme was determined, using 10% (w/v) sucrose, at a temperature ranging between 15 and 60 °C and a pH ranging between 3.5 and 7. The optimal pH and temperature for the immobilized enzyme were 5.5 and 25 °C, respectively. Immobilization of re-INVB on Nylon-6 showed no significant change in the optimal pH, but a difference in the optimal temperature was evident, as that for the free enzyme was shown to be 40 °C. The values for kinetic parameters were determined as: 984 and 98 mM for $$ K_{m}^{\text{app}} $$ of immobilized and free re-INVB, respectively. $$ K_{\text{cat}}^{\text{app}} $$ values for immobilized and free enzymes were 6.1 × 102 and 1.2 × 104 s−1, respectively, and immobilized re-INVB showed $$ V_{\max }^{\text{app}} $$ of 158.73 μmol h min−1 mg−1. Immobilization of re-INVB on Nylon-6 enhanced the thermostability of the enzyme by 50% at 30 °C and 70% at 40 °C, when compared to the free enzyme. The immobilization system reported here may have future biotechnological applications, owing to the simplicity of the immobilization technique, the strong binding of re-INVB to the support and the effective thermostability of the enzyme.
Starting Page	1289
Ending Page	1295
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	35
Issue Number	11
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-08-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Enzyme immobilization Invertase Nylon-6 Sucrose Zymomonas mobilis Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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