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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fukuda, Masaharu Kuga, Kazufumi Miyazaki, Ayako Suzuki, Tohru Tasei, Keito Aita, Tsunehiko Mase, Masaji Sugiyama, Makoto Tsunemitsu, Hiroshi |
| Copyright Year | 2012 |
| Abstract | A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine coronavirus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 102, 100, 101, and 102 TCID50/ml, respectively, and that for GBR was 106 copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle. |
| Starting Page | 1063 |
| Ending Page | 1069 |
| Page Count | 7 |
| File Format | |
| ISSN | 03048608 |
| Journal | Archives of Virology |
| Volume Number | 157 |
| Issue Number | 6 |
| e-ISSN | 14328798 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2012-03-11 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Virology Infectious Diseases Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Medicine |
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